To investigate effects of Paeonia Radix Rubra aqua-acupuncture on Adjuvant Athritis in rats, the edema rate, the number of WBC, the quantity of total protein, albumin and globuline in the blood serum were measured and histological test of the muscular tissue were done in the arthritis part.
1. After arthritis of Sprague dawley(SD) rats was induced by injecting Freund´s complete adjuvant for 2 weeks, normal saline was injected for the Control group and Paeonia Radix Rubra acua-acupuncture was injected for the Exp. group during 30 days. Selected point was Du´bf(ST_(35)) in both groups. And then the inframmator volume of plantar were checked. The volume of the plantar was 0.84 ± 0.14㎜ in Control group and 0.57±0.09㎜ in Exp. group, the swelling of plantar was restricted significanily in Exp, group(P<0.05).
2. The number of WBC was 10.34±0.14(10³/㎖) in normal group and 37.47±5.46(10³/㎖) in Control group. It was 25.21±3.45(10³/㎖) in Exp. group. This fact showed that Exp group. with Paeonia Radix Rubra acua-acupuncture was more effective than Control group in the treatment of arthritis(P<0.05).
3. The content of total protein in the blood serum were 6.14±0.93g/㎗ in normal group, 7.95±0.94g/㎗ in Control group, and 6.82±1.23g/㎗ in Exp. group. There was no significance in total protein between Exp. group and Control group from the statistical analysis.
4. The contents of albumin in the blood serum were 2.94±0.13g/㎗ in normal group, 2.01 0.48g/㎗ in Control group and 2.75±0.14g/㎗ in Exp, group. This fact showed that Exp. group had significant increase in the serum albumin from the statistical analysis, in comparison with Control group(P<0.05).
5. The contents of globulin in the blood serum were 3.19±0.48g/㎗ in normal group, 4.70±1.26g/㎗ in Control group and 3.58±0.73g/㎗ in Exp. group. There was no significance in the serum globulin between Exp. group and Control group from the statistical analysis.
6. In histological examinadon, because inflammatory reaction was active, remarkably irregular tissue and large amount of inflammatory cells were found in Control. group. But Exp. group showed that tissues were in some degree recovered, the number of inflamed cells was decreased and inflammation was restrained.
From these results, it is shown Paeonia Radix Rubra aqua-acupuncture restrained inflammatory reaction and muscular tissue necrosis in SD rat´s plantar was restrained by Freund´s complete adjuvant.
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